Critically Asses the Views of Paul Tillich on Religious Language

Critically Asses The Views Of Paul Tillich On Religious Language Paul Tillich was a renowned American Protestant theologian born in Prussia 1886. As a self-proclaimed philosophical theologian, Tillich saw the very nature of Christian faith expressed in religious symbols that demanded constant reinterpretation. He was famous for believing that it is possible to speak meaningfully about metaphysical concepts and therefore came up with the theory that religious language, because it is symbolic in nature, has an overwhelming effect upon humans.Tillich argued that religious language is symbolic. This means that religious symbols communicate the most significant values and beliefs of human beings. In his theory, Tillich firstly establishes the difference between signs and symbols and he does this by saying signs are something that point towards a statement and have no other effect as this therefore means that without understanding that sign, it is meaningless to you. An example would be a road sign indicating that you can now travel at the national speed limit and has no other effect of meaning.On the other hand, symbols possess much more meaning and power according to Tillich as they are actually involved and take part in what they are symbolising thus having an impact on it. For example the Cross that represents Christianity, Not only does it stand as a marker for that religion, but it also makes a powerful statement. It immediately reminds Christians of the sacrifice they believe Jesus to have made on the cross for them; it also reminds them of their beliefs about God and his plan for the salvation of human beings.After the distinction between the two had been made, Tillich claimed that religious language worked as a symbol for those who use it as it has meaning and impact on what it represents. He maintained that religious faith is best expressed through symbolism because a symbol points to a meaning beyond itself and best expresses transcendent religious beliefs . He believed that any statement about God is symbolic and participates in the meaning of a concept.Tillich famously used the example of a national flag to illustrate his point: a flag points to something beyond itself, the country it represents, but also participates in the meaning of the country. He believed that symbols could unite a religious believer with a deeper dimension of himself as well as with a greater reality. He also believed that symbols must emerge out of an individual collective unconsciousness, and can only function when they are accepted by the unconscious.For Tillich symbols cannot be just invented, but instead live and die at the appropriate times. Also Tillich suggests that religious faith, can express itself only in symbolic language, because â€Å"whatever we say about that which concerns us ultimately†¦ has a symbolic meaning† presumably because it is of greater concern and import than the mere language, which can only point towards it. â€Å"T he language of faith is the language of symbols†Tillich then went onto develop his idea of a symbol further by outlining the functions in which a symbol carries out which are Point to something beyond themselves, Participate in that to which they point, Open up reality that otherwise are closed to us and finally They also open up the levels and dimensions of the soul that correspond to those levels of reality. Tillich furthermore argued that symbolic language operates in a similar way that a piece of music, art work or poetry might.This is because they can heave a deep overwhelming effect upon us that we can’t explain or can only explain in a limited way and therefore, the person listening will not understand the effect unless they have felt the same effect of the same piece of art. Also, symbols, like artwork can open up new levels of reality for us and offer a new outlook on life that we would not previously of had, without looking at the symbol/art. Tillich Finally t alked about how religious language acts as a symbolic way of pointing towards the ultimate reality, the vision of God which he called ‘Being-Itself. Being-Itself is that upon which everything else depends for its being and Tillich believed that we came to knowledge of this through symbols which direct us to it. One critique of Tillich is the English philosopher of religion and theologian John Hick who argued that Tillich does not make clear how the symbol participates in that to which it points and claims in failing to do so produces a simplistic theory. Also he claims that there are religious statements which do not appear to â€Å"unlock dimensions and elements of our soul†, which is part of Tillich’s definition of a symbol.

Analysis of 3 Blind Mice Essay

I chose the story/nursery rhyme 3 Blind Mice to provide my summary and analysis on. It goes as follows – â€Å"3 Blind Mice, 3 Blind Mice†¦ See how they run, See how they run†¦ They all ran after the farmer’s wife, Who cut off their tails with a carver’s knife, Have you ever seen such a thing in your life? As 3 Blind Mice†. To summarize the above nursery rhyme I was able to conclude there are 3 mice and each mouse is blind. I found that the mice run and chased the farmer’s wife. I also was able to conclude that the farmer’s wife cut off all 3 of the mice’s tails. That is all I was able to come up with as far as summarizing goes. After completing an analyzation of 3 Blind Mice, I noticed symbolism, some unanswered questions and a few other findings I previously didn’t notice. I always assumed the farmer’s wife chopped off the 3 blind mice’s tails AFTER they were chasing her meaning she was defending herself. What if the wife cut off the 3 mice tails first and they were chasing her for attacking them initially? The lyrics does not advise which order either of the above 2 deeds occurred; it just states they both happened. It makes you question who the aggressor was, which may determine which side you are on and the lesson you learned from the story if any. After further analyzation, I began to wonder how the mice were able to chase the farmer’s wife around if they are blind. The author put extra emphasis on seeing the way the 3 blind mice run (those were the 1st 2 lines, the only lines that were repeated twice and the only lines that did not rhyme) as if it was different than how non-blind mice run. It’s unclear if the mice were running in fear, anger, if they were running different because they got their tails cut off etc. The author could be implying nothing good happens for people/animals with disabilities. The story seems to only highlight the mice’s deficiencies and nothing good happens for them. The last observation of my analysis is I never heard of a mouse, or even a few mice chasing a human in real life. Mice normally run from people in fear, not after them. I think symbolism was used to convey a message deeper than the simple nursery rhyme. Maybe the mice were used to represent the general public, and the wife represents their queen/leader. When the â€Å"little people† in society try to challenge the government or top authority figures, they normally get an example made out of them (hence the tail being cut off). It is unclear if the mice lived or died after their tails were chopped, but it’s clear they were punished by the farmer’s wife. Rodents were used to represent the â€Å"little people† because when rodents become a problem, you exterminate them!!! That is what I found when completing my summary and analysis for â€Å"3 Blind Mice†. I hope I was able to provide good theories to back up my findings while changing your view on an old nursery rhyme.

Response to Monets Water Lillies Thesis Example | Topics and Well Written Essays - 3000 words

Response to Monets Water Lillies - Thesis Example This commentary note is a series of reflections and important aspects of Clark’s experiences as he viewed two works by Poussin and wrote on his insight through these viewings, and on my own art-making, First, I discuss what insight the current body of literature concerning Monet’s Water lilies series can offer to a discussion of the work. Second, I examine the work through the perspectives presented in The Sight of Death and Art and its Objects. Third, I explore future research and lesson developments. Finally, I considered my reflections as they impact my future scholarship and art-making. While there are a host of famed artworks that grace the walls and spaces of the world’s great cultural centers and museums, few have gotten the attention of the public like those of the impressionists, particularly the works of Claude Monet (1840-1926). In fact, one of the highest prices paid for a single work of art was invested in one of his Water Lilies (1920-1926) series, which sold in 2008 for over $70 million. The interest in this series of paintings continues today, as seen by the public and critical response to the Monet’s Water Lilies exhibition held in New York City at the Museum of Modern Art from September 13, 2009 until April 12, 2010. The in-person access to artworks of this stature gives an opportunity for critique and interpretation from many viewpoints. It was in this spirit that Dr. Baldacchino granted permission for me to use my personal notes from several visits during this showing period in order to offer my interpretation of Water Lilies through a novel lens. Water Lilies In-class Presentation November 2010, Monet’s Water Lilies series is astounding in person. Its size is monumental, its colors are overwhelming, and its brushwork is beautiful. Upon my initial viewings, several key aspects of the work became apparent to me. First the monumental perspective of the three paintings seemed interesting to me because it stand s in contrast to many of the landscape paintings of the same time period. Second the brush strokes used by Monet in these works seemed expressive and natural, specifically in the water reflections and surroundings areas. Lastly, Monet’s unique style of brush stroke intrigued me. The Water lily triptych has a strong drawing quality about it that is also noticeable in many of Monet’s other artworks. He draws nature’s movement with his brush strokes rather than painting them. As a viewer felt, this technique gave me the effect of feeling closer to nature, as if having been face to face with elements of nature facing back at me. This water lily triptych created a romantic and natural atmosphere that caused me to become aware of the natural environment that I live in and appreciate this further. Considering Monet as naturalistic opens up a larger idea. As Temkin and Lawrence explain, â€Å"Artist’s ambition to create a panorama that enveloped the viewer, an e nvironment that in today’s parlance would be called an â€Å"installation†Ã¢â‚¬â„¢ (Temkin&Lawrence, p.9). As the High Museum states, these installations were sometimes large in size. â€Å"Monet created 250 different water lily paintings and 19 were large panels† (High Museum, 2009). The unique way he wanted to view these triptych paintings in the gallery space was interesting to me. As always, there is no indication of the horizon or pond’s edge, but here the viewer is treated to an exceptionally harmonious expanse of painted reverie. Softly flowing passages of cloud reflections, overhanging foliage, lily pads, and water share the space without dramatic incident (Temkin& Lawrence, p.34). The Water lilies panel caused two things to

See the prompt Essay Example | Topics and Well Written Essays - 1250 words

See the prompt - Essay Example ..† Machiavelli, p. 55. This quotation is from the book The Prince by Machiavelli 4. â€Å"Our love of what is beautiful does not lead to extravagance; our love of the things of the mind does not make us soft. We regard wealth as something to be properly used, rather than as something to boast about.† Pericles, p. 147 5. â€Å"The bourgeoisie, wherever it has got the upper hand, has put an end to all feudal, patriarchal, idyllic relations. It has pitilessly torn asunder the motley feudal ties that bound man to his natural superiors, and has left no other bond between man and man than naked self-interest, than callous cash payment† Marx, p. 738. This quote is from â€Å"Manifesto of the Communist Party, Chapter I â€Å"Bourgeois and Proletarians.† 1. When one lives the life away from the public eye, it is known as private life. Socrates reasons out his choice for living a private life that enabled him practice philosophy. He says that he has the divine umbr ella that protects and guides him for dos and don’ts in life. As for taking active part in life, he asserts that he has no divine directions. Socrates was too sincere, too honest as such he was unfit to be in public office and he would not be able to survive in the dishonesty prevailing over there. A public life is one that concerns and associates with the state. This area is engulfed with secular interests. According to the convictions of Socrates, if he were to take active interest in politics, in all probability it would not help him nor would it help the society. Personally his actions and responses are identical whether they concern the private or public life. One can control and discipline one’s private life, but as for public lifean individual is controlled by extraneous considerations and will have to make compromise with truth. 2. According to John Stuart Mill, self-will is one of the great misdemeanors of man. The barometer of goodness of humanity consists in obedience. A man has no choice but to act and perform. That which the human being does not consider as duty is a sin. Human nature is fundamentally corrupt as such redemption is only possible by killing human nature from within. With this perspective of life, demolishing any other human faculties, capabilities and vulnerabilities cannot be considered as evil. †¦ Man needs no capacity, but that of surrendering himself to the will of God, meaning the capacity to surrender itself is a great quality and liberty is kindergarten stuff as compared to the capacity to surrender. This is the Calvinistic theory. For man is the controller of all types of liberties. For an individual who knows the infinite power of surrender, individual liberty is of no consequence to him. Individual liberty is the demand by the imperfect individual. Total surrender is the domain of the perfect individual. 3. This book contains the guidelines for the dictators and tyrants to rule their subjects. Most of th e important rulers of Europe have benefited from the advice contained in the book and they helped to shape the governing policies. It has been hailed as the supreme guide of governance. The dictums prescribed by Machiavelli must have appealed to the baser instincts of such undemocratic rulers. He believed that the origin and substance of power was through wicked actions. He carefully distinguishes between getting and retaining power, which is done by wicked actions, but it is not possible to

Analysis Of The Tale Of The Veldt Essay Example | Topics and Well Written Essays - 750 words

Analysis Of The Tale Of The Veldt - Essay Example The traces "Happylife Home" along with the acquainted room adjustments similar to the parents' bedroom along with the nursery provide one with a feeling that this is a standard local house of those times. The mom appears concerned or even puzzled regarding something as she said "the nursery is...different right now compared to it was", this initiative can easily lead one towards thinking that mom is having ideal competencies to be a mother but when one reads on then it is revealed that she has the habit of demonstrating stereotyped reactions to every event that comes her way, the mother and father then state "nothing as well great for our children". Later in the story the mother and father talk about the issues of the amazing house and also the nursery, "The house is wife, mother, and nursemaid, Can I compete with it ?", along with the dad possesses a generic answer "But I believed that's exactly why we purchased this house". The mother and father in the tale appear upon their childr en's needs since solutions rather than means of revealing any specific really like or treatment. In the tale we by no means understand something about the kids except for their obsession with the room, "I don't need to do anything, however, appear and pay attention and also odor; what more will there be to perform ?†. The story is all about human nature to remain discontented with everything after a short period of time. The mother in the tale is master at finding something wrong with everything in the house, life and even in her children. The female character in the story is a representation of the people who fail to see the beauty in their life while, dwelling about the negativities of the existence. They attach their happiness with certain goals and objectives and once they are fulfilled then they find something else to worry about and perhaps become a living example of those who want to be happy and satisfied tomorrow (Mattews, 75).

Charging Children as Adults Essay Example | Topics and Well Written Essays - 2000 words

Charging Children as Adults - Essay Example With matters dealing with criminal justice, the method is not much different. First, the issue has to be stated, then it is discussed, and conclusions are drawn. Where steps look like they may have been skipped, the research objectives are within the job description of solving crime, and methodology and data collection is done during the discussion of the issue if it has not been done already. The matter at hand for this discussion is that of charging children as adults for the crimes they commit. When children are charged as adults for crimes, it can leave a permanent mark on their record that they will never be able to outlive. It is believed by some that this method of criminal justice will serve as a warning to those who would otherwise do harm to others, saving untold numbers of people from harm and homicide. Others believe that the children who are sentenced to these kinds of charges are from states of disenfranchisement, with matters only made worse by the fact that where they once did not believe they would overcome their situations, they are now supported in their own conclusions, guaranteeing them a life of less opportunity than those who may commit the same crimes, but do not suffer the same consequences as a result of the advocacy they receive from their community. In conducting researching for issues in the criminal justice system, the two most common methods are quantitative and qualitative (Maxfield; Babbie, 23). Quantitative analysis is that which deals with concrete facts, or quantities.

THE IMPACT OF INTERNATIONAL TRADE ON ECONOMIC GROWTH Essay

THE IMPACT OF INTERNATIONAL TRADE ON ECONOMIC GROWTH - Essay Example International trade is promoted through appropriate strategies and strict observation of the trade patterns. This essay seeks to discuss the impact of international trade on the economic growth. According to a study by the OECD in 2003, the elasticity of international trade was found to be significant. Results from 73 low and middle-income countries in developing economies indicated that there is a strong correlation between international trade and economic growth (Peacock 2013). Inward developing economies tend to grow at a slower rate compared to outward-oriented developing economies. Average growth rate is significantly higher after the liberalization of trade than the period before the liberalization. International trade involving imports of immediate goods leads to diffusion of technology in an economy (Berdell 2002). Most of the studies tend to support the positive effects of international trade on economic growth. The static impacts of the international trade refer to the impr ovement in the social welfare with a fixed resource supply. Opening up the global market offers the chance of trading at international prices (Peacock 2013). Domestic consumers can buy cheaper imported goods. At the same time, producers have the chance to export goods to other markets at higher foreign markets. The comparative advantage in the international trade leads to specialization and improved quality delivery. This has caused an increment in the social welfare and output. Another impact of international trade on economy is the dynamic gain. This is the change in the structure of production that can be attributed to adoption of new technologies (Peacock 2013). This has also led to increased scales of production. Expansion of production through international trade leads to economics of scale and are mostly based on the comparative advantage. There has been expansion in production which is a response to the demands in the global market (Berg & Lewer 2007). This expansion has led to a decrease in the cost of production and accumulation of capital (Berdell 2002). This has had an overall effect of increasing employment levels. International trade has been known for its support in the technological spillovers among the economies involved. This has favored productivity. International trade transmits knowledge into international market. A world renowned economist, Paul Krugman, through an article in The New York Times suggested that competitive devaluation in the 1930s was different from the modern of currency wars and international trade policies. Several countries were dependent on the gold standard at the time. In the modern fiscal policies, mutual interventions are hard to accomplish. In the past, gold was worth more than the domestic currencies. The conventional liberal-market interventions are seen to have no effect. Currency interventions are perceived to be accomplishing very little. This has caused major economies to get tempted to devalue their economi es by printing more money. International trade affects economic growth. According to Paul Krugman, international fiscal policies affect the incentives offered by the central banks which in turn affect economic growth. According to economist Milton Friedman, the most acceptable measure of the fiscal policy is their economic effect and not interest rates. Milton believed that unwarranted government intervention was unwelcome in

Senses and the endocrine system Essay Example | Topics and Well Written Essays - 500 words

Senses and the endocrine system - Essay Example These abnormalities that occur sometimes have no effect on the systems while in other cases the effects are detrimental. One of the common seen abnormalities as people age is the formation of cataracts. Cataracts are a clouding of the lenses in the eye to where light becomes hard to pass through. As a result of the light not being able to penetrate, problems with vision occur. Cataracts can form in a person’s eyes as the result of multiple conditions. Exposure to ultraviolet light can cause proteins to denature, which can lead to the condition. Certain diseases such as diabetes have been known to cause this to occur. Eye trauma and genetic issues can also be accounted for with the development of cataracts (Goldstein, 2010). Cataracts are an abnormality that is forming which affects the visual field of the individual. This is because the lenses in eyes are biologically centered around having a clear lens that is adjusted by the retina to allow light in depending on the light le vel. Even though aging of biological components is inherent as humans age, the formation of cataracts is often caused or started by external or internal determinalistic factors that cannot be accounted for. As a result, these are biological factors, which represent an abnormality to normal human physiology.

Citigroup Crisis Management and Continuity Plan Essay

Citigroup Crisis Management and Continuity Plan - Essay Example The economic crisis in the recent years had a significant impact over the nation thus impacting the businesses of the Citigroup as well. The current research focuses on the responses of the organizational group to the economic crisis and to learn the concepts and use of business management continuity plans in the process. Citigroup and the Global Economic Recession: The global economic recession that hit the United States in the late 2008 and thereafter the entire world affected the nation’s gross domestic product, led to unemployment, decrease in purchases, and decline in investments (U.S. Economic Downturn Worsened at End of 2008, 2009). Root Cause of the Recession leading Citigroup to a Crisis Situation: With the enhancement of the economic slowdown in the United States, the Citigroup started getting affected and was encountered with the crisis of recession. One of the major crises that led to the crisis in the Citigroup was the decline in the demand level among the custome rs. When a financial slowdown was prevailing in the United States, people tended to invest less and save their funds. When the nation was encountered with the decline in the economic growth, customers feared the failure or lack of performance of the different companies that included Citigroup as well. With scarcity in the availability of funds in the economy of the country customers started saving and stopped investing or lending to the company that ultimately led to a crisis for the company (Beek, 2010; Cochrane, 2010, p. 34). Thus the two primary reasons that could be reflected as the root causes for the crisis in the Citigroup are the economic slowdown in the United States followed by the change in the customer outlook and behavior towards fearing the failure of companies to overcome the global crisis, leading to huge declines in the levels of demand. Citigroup before the Crisis: Before the global recession had hit the United States, Citigroup had been successfully performing its activities. Several innovative strategies have been encouraged within the group. Also, with greater expansion plans the company created several job opportunities (Our commitment is our promise, 2006). The year 2007, which was before the economic crisis, the company reflected positive results with respect to its international consumer related performances, wealth management at an international level, as well as operations of services in its different business divisions. However, the results for the banking and marketing segments of the group were not satisfactory. Too much of lending and written down values might have led to such a condition for the group. The revenue generation was thus affected although the customer growth was increasing. The volume of products and the operating expenses of the group also started rising from the previous years. Credit costs for the financial sector of the group also increased (Citigroup’s 2007 Annual Report on Form 10-K, 2007). Citigroup du ring the Crisis: During the global economic slowdown, the Citigroup suffered huge losses that primarily occurred as a result of increasing costs of credits and the intensifying economic recession. Inflation and increasing levels of unemployment, that were resultant from the global econom

Will economic reform along with political repression succeed result in Research Paper

Will economic reform along with political repression succeed result in the Democratization of china - Research Paper Example According to Rummel, democracy can be achieved by regular election; through each particular vote counts, thus leaders are chosen to represent the people and policies that governors are chosen. The right for every citizen to vote is a second way of democracy, and the third is the right for any citizen with the required qualification to vie for the highest office and the right to form political parties in a country. Rummel (Para. 5) further notes that, democratization â€Å"means that a people rule themselves through periodic elections of the highest leaders in which nearly all adults can participate for which offices they are eligible and under the rule of law.† In addition, democracy is sometimes associated with conditions such as the freedom of the media in criticizing the government, its leaders and the policies implemented. Secondly, there is the freedom of more than one political party to competing for power in a country. The third is that the most popular and elected lead er is the head of government. In addition, according to Hauss (Para. 6), democracy is important since it yields to peace in a country. Hence, there is stable peace in democracies as a result of tolerance, trust, cooperation, and reconciliation within a country especially when conflicts arise. The author adds that, just as democratization is important when achieved, it is also difficult to achieve it. The ethnic tensions that result to conflicting issues may create mistrust and intolerance that will yield to lack of cooperation. However, democracy should be effective for results to be evident. China and democracy According to Harding (Para. 3), the Chinese communist party (CCP), had shown signs of embarking on democracy reforms since some leaders of the party had endorsed some issues of democracy, thus showing signs of gradual rise of democracy. However, the Chinese communist party did not choose democratization as one of its strategies in the enhancement of stability in the politica l system. Therefore, direct elections are still limited to the lowest level and nominations are normally controlled by the party. In China, the freedom of non-governmental organizations is limited. In addition, the press in China is usually restricted to some information and so is the internet, thus indicating lack of freedom of the media, which is usually a priority in a democratic nation. The Chinese communist party has only been enhancing more forms of authoritarianism, thus evidently showing that the leaders of this party fear that pluralism may be a risky move in terms of political power and in the continuation of foreign policies, which, ironically, are viewed as of low value by the citizens. In addition, the middle class, which lead to the economic development, was said to be a driver in the promotion of democracy. However, the middle class usually demand for a voice in politics and in China, this group has been rewarded with high salaries, a political voice, and it is repres ented in the Chinese communist party. These have been the strategies used by the communist party to keep the middle class opposition low. Since the middle class members seem to have an interest in democracy, the country has to attend to their demands both politically and economically, so that they may loose their interest for democratization. The China’s middle class believe that the low levels of education and poverty in some part of the country would yield to unworthiness of democracy. Nevertheless, Harding (Para. 9) further argues that, in china for the past years it would have been easier to implement democratic mechanisms by abolishing the property

An analysis of the current activities of Cherwell District Councils Environmental Services Department Essay Example for Free

An analysis of the current activities of Cherwell District Councils Environmental Services Department Essay 1.0 Background Cherwell District Councils Environmental Services Department is made up of two sections (Environmental Services and Amenity Services), which deliver a wide range of diverse services (see Table 1). Table 1. Services offered by the Environmental Services Department. Environmental Services Amenity Services Food Safety/Health and Safety in businesses Waste Collection/Recycling Animal Welfare/Dog Warden Service Street Cleaning Pollution Control/Noise Nuisance Pest Control Service Health Promotion and Disability Issues Environmental Strategy/Agenda 21 This report looks at some of the services provided by the commercial team within environmental services who are responsible for food/health and safety law enforcement, infectious disease control, food/water sampling, licensing/registration, complaint investigation and offering information and training. 2.0 Introduction The majority of the activities of the commercial team are mandatory imposed on the Council by statute such as the Food Safety Act and the Health and Safety at Work etc Act. The sections activities are also monitored through quarterly reports to the Chief Executive of the Council, the Food Standards Agency and The Health and Safety Executive. Despite the regulatory function of the section which is primarily the enforcement of the law, it none the less provides a service which is described as the combination of outcomes and experiences delivered to and received by a customer (Johnson Clark, 2001 p9). Figure 1 lists some of the aspects of the service experience, which can be used when assessing the quality of the service provided. * the extent of personalisation of the process * the responsiveness of the service organisation * the flexibility of customer-facing staff * customer intimacy * the ease of access to service personnel or information systems * the extent to which the customer feels valued by the organisation * the courtesy

Social Work Practice: Values and Ethics

Social Work Practice: Values and Ethics Introduction At its most basic social work is a consistent and organised approach to the social problems of families and individuals. It is an approach that focuses on helping people to help themselves (Moore, 2002).[1]Most practitioners enter social work because of a commitment to social justice, or at the very least a desire to help others and to see improvement and positive change in people’s lives. Modern social work, along with other public services, is being increasingly run along business lines and market principles. Today’s social worker therefore is confronted with the managerial approach, performance indicators, care managers and care packages. Given the current state of things where everything is guided by budgets and the need to satisfy those in charge by processing a case as quickly as possible one wonders whether a social worker has time to be guided by general ethical principles, let alone a specific code of practice. In the current climate it is easy to forget that s ocial workers are committed to the view of the intrinsic human worth of each individual, and the concomitant view that each person deserves the best care and advice possible. In 1948 the Universal Declarati[2]on of Human Rights came into being. The declaration encapsulates a view of the inherent worth and dignity of the human person. The declaration espouses the notion of individual freedom on the basis that such freedom did not infringe the rights of others, these are the rights on which much of social work practice is based. This paper will begin with a general definition of ethics. It will then look at the ethics and values which underpin social work practice. There will be an assessment of social work values and of their relevance to anti-discriminatory and anti-oppressive practice. Ethics Ethics are general moral principles that are intended to inform the governing of human behaviour. Thus, most people would hold to the view that some things such as cruelty and murder are always wrong, or unethical. Ethical codes, however, may be based on moral principles, but are not as generalisable or universally applicable. This is because they refer to particular professions and the way in which professional behaviour is governed. Such ethical codes cannot be said to be neutral or value free because they are contextual. They arise within a certain context and are applied to a particular purpose as such, ethical codes are influenced by the ideologies held in that context (Butler, 2000). The context being examined here, is that of social work practice. Codes of practice and ethics are often idealistic, because they may be seen as providing a blueprint for how a social worker should act when it is impossible to legislate for every situation, and this may result in a false sense of s ecurity (Banks, 2003). Social workers therefore, need to be able to recognise when the code of practice within which they are required to work, does not operate within a framework that is informed by human rights and social justice (Husband, 1995). The Client’s Needs Shon (1991) has argued that: Professionals claim to contribute to social well-being, put their clients’ needs ahead of their own, and hold themselves accountable to standards of competence and morality. (Schon, 1991:11-12). Some critics maintain that the way in which social services often operates is self-serving rather than serving the needs of the clients, yet social workers do police themselves and their profession. The way in which they do this is to think critically about what they are doing, why they are doing it, and what moral implications this may have. Certainly social work ethics should not lead anyone to believe that the social work profession should serve itself, rather the needs of the client should be most important. One of the ways this is achieved is by establishing clear relationship boundaries early on. This is because involvement with a client that is too personal is contrary to what the BASW has to say about social work ethics and values. The social work profession promotes social change, problem solving in human relationships and the empowerment and liberation of people to enhance well-being. Utilising theories of human behaviour and social systems, social work intervenes at the points where people interact with their environments. Principles of human rights and social justice are fundamental to social work (BASW,2001). [3] Social work practice, in order to be ethical practice must be centred on the needs of service users Social workers of necessity intervene in people’s lives and have an influence on situations, ethical decision making is therefore a vital component of social work practice (Osmo and Landau, 2001). The Association is there to give advice to social workers on what constitutes ethical decision making in different contexts. Ethical Decision Making Decision making has to be grounded in the values and ethics of social work. Some of the issues that social workers have to deal with and that involve them in ethical decision making centre around balancing the rights of one individual against others, around public welfare and issues of institutional and structural oppression. This can make life difficult because the social worker has then to identify when institutions and structures are being oppressive and how the values of social work may be used to combat this. Decision making is also problematic because social workers are committed to confidentiality with respect to the people they work with. Sometimes a social worker is faced with a situation where he or she may, for the greater good, be forced to break the ethic of confidentiality because the client may be a danger to themselves of someone else. This causes a conflict of values and the social worker has, with the help of his/her supervisor, to balance the needs of the individua l against the needs of the greater good. At the very least social work should begin with a clear commitment to social justice which seeks to challenge poverty and discrimination in all its forms because social work has its roots in the nineteenth century moves to eradicate poverty and unemployment. Everyone has the right to be protected from abuse and to be treated with respect. This is not easy when Government discourses express concern for inclusion and equality e.g. for those with mental health problems, then employ discourses which ignore factors such as race, gender and class and social circumstances, that are pertinent to any proper understanding of a person’s condition. The Human Rights Act of 1998 makes it mandatory for local authorities to act in ways that are conversant with the Act. Social workers help with the problems faced by people with disabilities. Social workers have a duty to be conversant with the Human Rights Act and the Community Care Act of 1990. Social workers are faced with making decisions concerning what defines a person with disabilities and also how to assess their needs. If the wrong form of care is prescribed, e.g. detainment under the Mental Health Act for a person who does not fulfil the specified criteria, then this could be an infringem ent of human rights. Social workers are duty bound to base their practice on concepts of human rights and social justice but at the same time they need to be more aware of how the inequalities that they see in society might affect their practice (Cemlyn and Briskman 2003). Society does not always operate in the best interests of the individual person, furthermore, the introduction of market principles into social care can mean that the legal framework within which a social worker has to act may also (however much it is unintended) work against individual rights. Some commentators e.g. Challis (1990) maintain that prior to market principles and managerialism being introduced into social care, social workers operated with a much greater degree of freedom. It is arguably the case that the social worker is duty bound to act in accordance with a process that is informed by the valuing of the human person and the concept of human rights, and may, therefore, need to be prepared to work outside of a framework th at (albeit unintended) prevents them from working according to this ethic (Cemlyn and Briskman, 2003). Wolff (2002) speaks of virtue ethics being the root of social work practice because of its concern with a just society and justice for individuals. Bearing this in mind social workers seek to engage in anti-discriminatory and anti-oppressive practice in all that they do. Anti-discriminatory and Anti-oppressive Practice An emancipatory and anti-oppressive attitude is a critical component of ethical social work, social workers should be people enablers, enabling people to stand up for their rights and giving them a voice. Horton and Pattapen (2004) argue that in contemporary society individuals are often disempowered in numbers of ways and feel unable to cope with the rules that guide their lives. Social workers deal with the distress that results from this and in their practice should question social systems wherein an increasing number of people suffer from injustice, oppression, and exclusion from mainstream society. Anti-discriminatory practice means taking account of structural disadvantages i.e. how the structures of society often work against certain groups e.g. those with disabilities, women, and ethnic minorities. It also means that the social worker takes care not to use discriminatory language and to do their best to promote the dignity and self-worth of service users. The ethos of the worth of individual human persons is often counteracted by current social work practice. Under the guidelines for social care social workers help individuals to choose what is best for them, in practice however, care managers are the people who hold the budgets and budgetary concerns can often be discriminatory disenfranchising the person that the social worker is trying to help (Postle, 2000). Social workers try to avoid this happening but they work in a state institution that often inadvertently works against the best interests of service users. This is why social workers need to be trained to view the world from the perspective of others so that they more easily recognise how the system oppresses (Moore, 2002).. Conclusion The ethics and values that underpin social work practice are dedicated to social justice and recognition of the inherent worth of each and every individual. Social workers do their best to work in partnership with service users and to do this in a way that includes and empowers people. Social workers are dedicated to help people move forward and take control of their lives, the idea is to shift the power balance away from the social worker and towards the person that needs help. The structures of society are, in some ways, discriminatory and oppressive and social workers recognise that white masculine values disadvantage people and they are therefore committed to anti-discriminatory and anti-oppressive practice in their work. Bibliography Banks, S. 2003 From oaths to rulebooks: a critical examination of codes of ethics for the social professions European journal of Social Work Volume 6 No. 2 July 2003 p. 133-144 British Association of Social Workers (2002) The Code of Ethics for Social Work.http://www.basw.co.uk/. Butler, I 2000. A Code of Ethics for Social Work and Social Work Research http://www.elsc.org.uk/socialcareresource/tswr/seminar6/butler.htm Cremlyn, S and Briskman L. 2003 â€Å"Asylum, Children’s Rights and Social Work† Child and Family Social Work 8 (3) pp. 163-178 Husband, C. (1995) The morally active practitioner and the ethics of anti-racist social work. In: Ethical Issues in Social Work (eds R. Hugman D. Smith), pp. 84–103. Routledge, London Ife, J. (2001) Human Rights Social Work: Towards Rights-Based Practice. Cambridge University Press, Cambridge Postle, F. (2000) The social work side is disappearing. I guess it started with us being called care managers, Practice, 13(2), pp. 13-27. Ring, C. 2001 â€Å"Quality assurance in mental-health care: A case study from social work† Health and Social Care in the Community 9(6) 2001 pp. 383-390 Schà ¶n, D. A. (1991) The Reflective Turn: Case Studies In and On Educational Practice, New York: Teachers Press, Columbia University Slote, Michael. From Morality to Virtue. Oxford: Oxford University Press, 1992 Wolff, J. 2002 â€Å"Contractualism and the virtues† Critical Review of International Social and Political Philosophy Volume 5 No. 2 June 2002 p. 120-132 1 Footnotes [1] See p.179 [2] [3] http://www.basw.co.uk/articles.php?articleId=2page=2

Sucrose Synthase Key Enzyme In Sucrose Metabolism Biology Essay

Sucrose Synthase Key Enzyme In Sucrose Metabolism Biology Essay Sucrose synthase is a key enzyme in sucrose metabolism. Sucrose metabolism is required by the plant to form carbon required for various processes in the plant such as respiration, starch and cell wall formation. The enzyme is encoded by a small multigene family where most plants have at least two isoforms of the enzyme. The kinetics of sucrose synthase show that different Km values and ratios of sucrose breakdown exist for the enzyme. The methods of extracting, assaying and purifying the enzyme are shown in the enzyme characteristics. Factors such as pH, addition of different buffers, metal ions, fungal volatiles as well as environmental factors such as anoxia have all been shown to affect sucrose synthase activity. The enzymes protein sequences have been phylogenetically divided up into six main groups using clustalw. Sucrose synthase is normally present in the cytoplasm but the availability of sucrose in the chloroplast and its ability to use ADP as a substrate would indicate that the enzyme may be able to act in the chloroplast as well as the cytoplasm. Sucrose synthase is an important enzyme in sucrose metabolism in plants cells. (Persia et al., 2008) The main route of entry of carbon from sucrose is commonly known to be sucrose synthase. (Bieniawska et al., 2007) This carbon is used for respiration and in the synthesis of cell wall polymers and starch. (Persia et al., 2008) The main form of reduced carbon in plants is sucrose. It is used to support growth and synthesis of reserve materials e.g. starch in heterotrophic sink tissues. (Matic et al., 2004) The UDP-glucose supplied by sucrose synthase is used for cell wall biosynthesis while working with the cellulose synthase complex. (Baud, Vaultier and Rochat, 2004) In most fruit tissues, an increase in sucrose synthase activity is alongside with sucrose accumulation. This would suggest that sucrose synthase plays a physiologically important role. (Islam, Matsui and Yoshida, 1996) Carbohydrates are transported from photosynthetic source tissues to sink tissues in the form of sucrose . The consequent cleavage of sucrose in the sink tissues is the first step for its use in various metabolic pathways. The sugar is cleaved in vivo by either sucrose synthase (Sus) or by invertase. Invertase catalyses an irreversible reaction where sucrose is cleaved into glucose and fructose (Matic et al., 2004) while sucrose synthase catalyses the reversible conversion of sucrose and uridine-diphosphate (UDP) into uridine-diphosphoglucose and fructose. (Hirose, Scofield and Terao, 2008) (Hardin and Huber, 2004) These enzymes play a crucial role in plant growth and development. (Abid et al., 2009) Sucrose Synthase is cytosolic (Ã…  ebkovà ¡ et al., 1995) and has been characterized in many different plant species such as maize (Hardin and Huber, 2004), rice (Odegard, Liu and Lumen, 1996) and sugarcane (Schà ¤fer, Rohwer and Botha (2005)). Its activity has been studied in many plant organs such as roots, leaves and seeds. (Ã…  ebkovà ¡ et al., 1995) For trees, cellulose biosynthesis is a highly regulated process in which carbon is permanently placed in their primary and secondary cell walls. Sucrose is the main carbon source for cellulose synthesis. The stem is made up of extremely active sink cells which utilise sucrose for cellulose synthesis. Sucrose synthase is the main sucrolytic enzyme in these cells that catalyzes the reversible conversion of sucrose into fructose and UDP-glucose which is needed for cellulose biosynthesis. (Joshi, Bhandari and Ranjan, 2004) It also plays an important role providing adequate sugar supply during anoxic stress. It has been shown that during anoxic germination of rice, sucrose synthase activity was enhanced whereas the activity of invertase was depressed. This would indicate that sucrose synthase is the enzyme predominantly responsible for sucrose breakdown during anoxia. (Joshi, Bhandari and Ranjan, 2004) Fig 1: Diagram of the cleavage and synthesis reaction of sucrose synthase (Rà ¶mer et al., 2004) Different isoforms of the gene are present in most plants. In the case of maize, two non-allelic genes were discovered for sucrose synthase but more investigation lead to the discovery of a third. At least three genes for sucrose synthase have been discovered in rice where the genes show differences in expression between tissues. RSus1 is expressed in root phloem while RSus2 is expressed in leaf phloem. (Schà ¤fer, Rohwer and Botha, 2005) When examining the different isoforms at an amino acid level it is appears that there is less homology between different sucrose synthase genes in a species than when the gene is compared to its corresponding gene in another species. In the case of maize, there is 75% homology between the SS1 gene and SS2 gene of maize but there is 90% homology between rice RSus1 and maize SS2 genes. In sugarcane, the SS1 gene is 97% identical at the amino acid level to maize SS1 gene. (Lingle and Dyer, 2001) Nolte and Koch (1993) undertook a study to determine whether sucrose synthase was localized to certain part of the vascular strand. It is well known that sucrose synthase is present in vascular bundles for example in transgenic tobacco plants phloem specific expression of a maize sucrose synthase gene has been observed. Their study, using immunohistochemistry, found that sucrose synthase was restricted to the cytoplasm of companion cells of the phloem and did not appear to be present in other organelles of the plant. (Nolte and Koch, 1993) The molecular mass of sucrose synthase can be determined by gel filtration. Sucrose synthase elutes from the column with a Kav value of 0.17844 which when using a calibration curve correlates to a molecular mass of 362kDa. Using SDS-PAGE gradient gel the molecular mass of each subunit can be estimated at 92kDa. This can conclude that sucrose synthase is a tetrameric enzyme with a molecular mass of 360kDa and four identical subunits of 90kDa. (Hardin and Huber, 2004) (Elling and Kula, 1993) It can associate with membranes and the actin cytoskeleton where its activity is known to be involved with cellulose synthesis. It does this by channelling uridine-diphosglucose to the growing glucan chain by the enzyme cellulose synthase. (Hardin and Huber, 2004) Analysis of Sucrose Synthase Gene Family: From the results of species examined to date, it is shown that sucrose synthase is encoded by a small multigene family. (Bieniawska et al, 2007) Most species of plants have at least two isoforms of sucrose synthase. These isoforms usually have comparable biochemical properties and highly homologous amino acid sequences. (Wen et al., 2010) Further analysis of transgenic and mutant crop plants show certain isoforms of sucrose synthase have specific functions in the plant. The rug4 mutation of pea removes the SUS1 isoform but has no effect on SUS2 or SUS3. This would indicate that these two isoforms are not able to make up for the loss of SUS1 in the seed or root nodule. It is clear that the loss of different isoforms affect the plant in certain ways. Loss of the SH1 isoform in maize has different outcomes from the loss of SUS1 isoform. SH1 is required for normal cell wall formation during endosperm development while both isoforms are needed for wild-type rates of starch synthesis. Why different isoforms have different functions is unclear. The same functions can be carried out in the cell by different isoforms but can occur in distinct cell types, developmental periods or environmental conditions. It is likely that different isoforms could have non-overlapping, particular functions in the same cell. (Bieniawska et al., 2007) It is difficult to decide on the precise roles of the genes in sucrose synthase gene family when there is not enough information in existence. Although there is some information available on some of the isoforms and theyre functions in the plant, no analysis of the functions of the gene family has been carried out. The model plant Arabidopsis is ideal for carrying out such an analysis. Six sucrose synthase genes are in the Arabidopsis genome. Based on comparisons of the amino acid sequences the isoforms they encode can be divided into three distinct pair groups. The isoforms SUS1 and SUS4 are 89% identical to each other but have less than 68% similar amino acid sequences to other isoforms. Similarly, SUS2 and SUS3 are 74% identical to other isoforms and are 67% less identical to the other forms of enzyme. SUS5 and SUS6 are 585 identical to each other but have less 48% similarity to the other isoforms. When examining other dicotyledonous species it appears that at least two of the thr ee pairs of isoforms are present. When phylogenetic analysis was carried out, it showed that the isoforms AtSUS1 and AtSUS4 are related to pairs of isoforms from pea (Fabacae), carrot (Umbelliferae) and potato (Solanacae). A pair of isoforms from Craterostigma plantagineum (Scrophulariacae) is closely related to the pair of isoforms AtSUS2 and AtSUS3 in the Arabidopsis. The pair AtSUS5 and AtSUS6 is related strongly to a pair of genes from rice. This evidence shows that it is unlikely that the three pairs of isoforms in Arabidopsis are as a result of gene duplication events. It is possible that each isoform has an exact function preserved in a wide range of plants. The members of Arabidopsis gene family are strongly differentially expressed in different organs of the plant through its development and in response to external stimuli e.g. environmental stress. This is seen in gene families of other plants studied. (Bieniawska et al., 2007) Fruit quality is determined by the type and quality of sugars present. A study of the sucrose synthase-encoding gene from the muskmelon fruit was carried out to evaluate how to genetically improve the quality of the fruit. This is done by finding the sugar components in fruit, to identify the enzymes involved in sugar metabolism and distinguish the relationship between sugar accumulation and the activities of related enzymes. It is thought that sucrose synthase is the enzyme involved in metabolising sucrose in developing muskmelon fruit. To examine this, a full length cDNA strand encoding sucrose synthase was extracted from a muskmelon fruit by RT-PCR and RACE and identified as CmSS1. Real time PCR analysis showed that CmSS1 expression changed in among different tissues of the plant e.g. root, stem, leaf. It showed that the mRNA levels are highest in the root and lowest in mature fruit. Fig 2: The patterns of CmSS1 transcript abundance in the different tissues of the muskmelon plant. These results were found using quantitative real-time PCR analysis of total RNA prepared from the root, stem, leaf, flower and mature fruit of muskmelon. During fruit development and ripening it was shown that CmSS1 mRNA was at its maximum level at five days after pollination and decreased steadily during fruit development until it reached its minimum level of maturity. This was discovered using again real-time RT-PCR analysis of mesocarp tissues from five days of pollination to ripening. Fig 3: This graph depicts the patterns of CmSS1 transcript abundance in developing muskmelon fruits found by using quantitative real-time PCR analysis of total RNA prepared from muskmelon. (Wen et al., 2010) The sugar content and SS activity were analysed to show the functions of CmSS1 in regulating fruit quality. It showed that very low concentrations of sucrose are present in young and unripe muskmelons. Between 20 and 30 days after pollination there is a massive rise in the amount of sucrose in the fruit. Sucrose synthase activity increased in the direction of sucrose synthesis and decreased in the direction of sucrose cleavage through fruit development. (Wen et al., 2010) Fig 4: The depiction of sucrose content and sucrose synthase activity during muskmelon fruit development. The first chart shows sucrose content during fruit development. The second shows sucrose activity in the sucrose synthesis direction and the third shows sucrose cleavage direction during muskmelon fruit development. (Wen et al., 2010) Enzyme Kinetics of Sucrose Synthase: An investigation was carried out by Schà ¶fer et al. to the find the properties of three sucrose synthase isoforms present in sugarcane. Kinetic analysis indicated that the three sucrose synthase genes in sugarcane are different isoforms, with major differences in Km values and the ratios of sucrose breakdown synthesis. The kinetic characteristics of the SuSyA and SuSyB isoforms, both expressed in the leaf roll, differ greatly. It was found that SuSyA has almost three times higher affinity for sucrose than the SuSyB isoform whereas SuSyB has a much greater affinity for UDP than SuSyA. Based on the differences in their kinetic properties it can be concluded that SuSyB and SuSyC are different isoforms of sucrose synthase. SuSyC has roughly ten times higher affinity for UDP compared to the other two isoforms. (Schà ¤fer et al., 2005) Fig 5: The graph shows the Lineweaver-Burk plot of 1/v against 1/S for the isoforms SuSys A, B and c where UDP was the variable substrate. The concentration of sucrose was kept constant at 320nM. The Km values were determined from the non-linear fit of the data to the Michaelis-Menten equation. (Schà ¤fer et al., 2005) When examining sucrose synthase in soybean nodules Morell and Copeland (1985) found the kinetic constants of UDP, UDPglucose, sucrose and fructose by fitting the data to the following two equations: 1. v = VA/KiaKh + KhA + KhB + AB 2. v = VA/Ka + A + A/Ki The kinetic constants for ADP, CDP and ADPglucose were found using non linear regression analysis of initial velocity data. Fig 6: Graph showing the effect of sucrose concentration on the cleavage activity of sucrose synthase in soybean nodule. The lines show the fit of data to equation 1. The reaction mixture were composed of 20 µmol Hepes-KOH buffer (pH 7.5) 2 µmol UDP, 1.5 µmol NAD, 25 µg UDPglucose dehydrogenase. Each symbol represents a different concentration of sucrose. The dark circle shows 3.2 µM, the clear circle shows 4 µM, the dark triangle shows 6.25 µM, 10 µM is shown by the clear triangle and the dark square depicts 20 µM. In the cleavage and synthesis direction standard Michaelis-Menten kinetics are observed. The variation of concentration of sucrose at different concentrations of UDP gave an intersecting pattern of linear double reciprocal plots. (Morrell and Copeland, 1985) Parameter Value V (U/mg protein) 13.3 ±2.0 Km sucrose (mM) 31.3 ±7.1 Ki sucrose (mM) 31.9 ±13.1 Km UDP (mM) 0.005 ±0.002 Ki UDP (mM) 0.005 ±0.001 Fig 7: Table showing the kinetic parameters for the cleavage reaction of sucrose synthase in soybean nodules. (Morrell and Copeland, 1985) Fig 8: The graph depicting the effect of UDPglucose concentration on the synthesis reaction of sucrose synthase activity in soybean nodules. The reaction mixtures contained 20 µmol Hepes-KOH buffer, 15  µmol fructose, 5 µmol MgCl2, 0.4  µmol P-enolpyruvate, 0.15  µmol NADH, 20 µmol KCl, 25 µg pyruvate kinase 25 µg lactate dehydrogenase and the required amount of enzyme. As in the previous graph, the amount of UDPglucose was varied in the presence of 2.5mM (dark circle), 3.2mM (clear circle), 4mM (dark triangle), 5mM (clear triangle) and 8mM (dark square) fructose. The results on the graph are representing the fit of data to equation 1. When the concentration of UDPglucose was varied at the concentrations of fructose in the graph, an intersecting pattern of linear double reciprocal plots was seen. From fitting the data from the graph to equation 1, it is noted that substrate inhibition would have occurred at a concentration greater than 15mM fructose. Parameter Value V (U/mg protein) 14.3 ±1.2 Km fructose (mM) 3.7 ±0.8 Ki fructose (mM) 19.6 ±9.9 Km UDPglucose (mM) 0.012 ±0.006 Ki UDPglucose (mM) 0.064 ±0.014 Fig 9: table showing the kinetic results by fitting the figures from the graph to equation 1. When partially purified SuSyA, SuSyB and SuSyC were blotted to a nitrocellulose filter the results showed that all three isoforms are approximately 94kDa. (Schà ¤fer et al., 2005) The would correlate to the findings of Hardin et al and Lothar et al who stated that sucrose synthase is tetrameric enzyme made up of four 90kDa subunits. Fig 10: Immunoblot of sugarcane SuSy. A crude extract of protein from leaf roll was loaded into lane 2 while partially purified isoforms of SuSyA, SuSyB and SuSyC were loaded to lane 3, 4 and 5. The molecular weight ladder was used to identify the bands see in each lane. (Schà ¤fer et al., 2005) Characteristics of Sucrose Synthase: Extraction of Protein: The method for extracting protein from the leaves of maize (Zea mays), rice (Oryza sativa) and tobacco was done as follows: 1-3g of leaves was ground in liquid nitrogen and the powder was mixed in the ratio 1:2 with extraction buffer. The buffer was made up of 0.1M tris-HCl, pH 8, 10mM DTT and 1% polyvinylpolypyrrolidone. The samples were then incubated on ice for 15 minutes and then centrifuged at 1,000g for 10 minutes at 4oC. The pellet was then removed and the supernatant was re centrifuged at 100,000g for one hour at 4oC. After this final centrifugation, the pellet and supernatant which contained the soluble proteins was resuspended in sample buffer for electrophoresis. (Persia et al., 2008) When extracting protein from rice seeds, a similar procedure is followed. Seeds weighing roughly 50-100mg at various stages of growth were homogenized in 400 µl of extraction buffer and kept at 4oC. The buffer was made up of 50mM Tris-HCl, pH7.5, 1.0mM DTT, 1.0mM EDTA and 2mM PMSF. Ammonium sulphate fractions (30-50% w/v) were precipitated and then resuspended in dialysis buffer made up of 50mM Tris-HCl, pH 8.0, 5mM MgSO4, 5mM 2-mercaptoethanol. This was then dialyzed overnight at 4oC. (Odegard, Liu and De Lumen., 1996) The method for extracting protein from tobacco pollen tubes is slightly different to those mentioned previously. The pollen first was slowly thawed from storage at -20oC and hydrated in a humid chamber overnight. It was then germinated in BK medium and allowed to germinate at 25oC for three hours. After this period had elapsed, the pollen was collected by centrifugation at 1,000g for 5 minutes at 25oC. It was then washed twice with BRB25 buffer which is made up of 25mM HEPES, pH 7.5, 2mM EGTA and 2mM MgCl2 and 15% Suc. After washing, the pollen was resuspended in lysis buffer and lysed on ice using a motor-driven Potter-Elvehk-jem homogenizer. The lysis buffer used was made up of BRB25 buffer along with 2mM dithiothreitol, 1mM phenylmethylsulfonyl fluo ride (PMSF), 10 µL/mL protease inhibitors, 1mM NaN3 and 10% mannitol. After lysis was carried out, the samples were centrifuged at 1,000g for 10 minutes at 4oC. The supernatant was centrifuged again at 4oC for 45 minutes at 100,000g over a 20% (w/v) Suc cushion. The supernatant was then collected as it contained the soluble protein fraction. (Persia et al., 2008) Enzyme Assays: After extracting protein, the sucrose synthase activity in sugarbeets was found using a spectrophotometric end point assay. The activity of the enzyme was monitored as fructose formed at 35oC. This was carried out in a solution that contained 250mM sucrose, 2mM UDP and 100mM MES. The control was carried out by assaying for activity in the absence of UDP. The total protein concentration was determined using the Bradford method where bovine serum albumin was the standard. (Klotz and Haagenson., 2008) When assaying for protein from rice, the Bradford method was followed to determine protein concentration as was done in Klotz et al. 40mg of protein was used per assay. The assay was carried out in 20mM MES pH 6.4, 200mM sucrose and 4mM UDP for 15 minutes at 30oC. The reaction was stopped by boiling for 2 minutes and the fructose levels were measured. The control tubes did not contain UDP. (Odegard, Liu and De Lumen., 1996) When examining the effect of sucrose synthase on carbon partitioni ng a similar method was followed for assaying the protein. Sucrose synthase was assayed in the direction of sucrose breakdown using 50 µl poplar plant extract. The tetrazolium blue assay was followed to determine the amount of free fructose. As in previously mentioned assays, the absence of UDP in the assay acted as a control. The total protein content was found by employing the Bradford (Bio-Rad) protein assay. (Coleman, Yan and Mansfield., 2009) A similar method was followed for carrying out an assay for the enzyme on tomato tissue. The reaction mixtures contained 50mM Hepes-NaOH buffer, 15mM MgCl2, 25mM fructose and 25mM UDP glucose. This was incubated at 37oC for 30 minutes and was terminated with the addition of 70 µl of 30% KOH. The enzyme blanks were terminated with the addition of KOH at 0 minutes. The tubes were then kept at 100oC for 10 minutes to destroy any fructose. The soluble protein content was determined using the Lowry method whereby bovine serum albumin was th e standard. (Islam, Matsui and Yoshida., 1996) Alkaline copper solution is added to each tube and allowed to stand at room temperature for roughly 30 minutes. Dilute folate reagent is then added to each tube rapidly and after 30 minutes the absorbance is read at 750nm. (Lowry et al., 1951) The results were measured as  µmole of sucrose per minute per mg protein. (Islam et al., 1996) When assaying for sucrose synthase in the cleavage direction Rà ¶mer et al used recombinant SuSy1 gene from potato. In a volume of 100 µl HEPES buffer with a concentration of 200mM and pH 7.6 recombinant sucrose synthase was incubated along with 2mM UDP and 500mM sucrose for ten minutes at 30oC. HPLC analysis was used for the formulation of UDP-glucose. The Bradford assay was used to determine protein concentrations as was carried out by Klotz et al and Coleman et al. The activity of the enzyme was also tested with the nucleoside diphosphates dTDP, CDP, ADP and GDP at 2mM. For assaying recombinant e nzyme in the synthesis direction a similar method was followed as when assaying for standard enzyme. Recombinant sucrose synthase was incubated in a total volume of 100 µl HEPES buffer where this time the pH was 8.0 and the concentration was as in cleavage direction of 200mM. 1mM UDP-Glc and 20mM D-fructose was also added to the mixture and it was incubated for five minutes at 30oC. The reaction was heated to 95oC for five minutes and HPLC analysis was used to establish the formation of UDP. The sucrose synthase activity was also tested using dTDP-Glc, CDP-Glc and ADP-Glc. (Rà ¶mer et al., 2004) Purification of Protein: After extraction of the protein from the crude extract, purification can be carried out. This can be done in a number of ways such as Batch adsorption with Sephadex A50, Anion exchange chromatography and Gelfiltration. SDS-PAGE can be carried out after purification to check the purity of the protein sample. The Sephadex A50 gel is loaded into a glass funnel and washed twice with deionised water. The gel was then washed twice with 300ml standard buffer. The protein sample was loaded to the gel and slowly sucked through the gel for 30 minutes. The gel bed was then washed with 300ml standard buffer and then with 300ml standard buffer containing 100mM KCl. The last washing step contained 300mM KCl. 200ml of the first salt preparation was concentrated to 40-50ml by using a cross-flow ultrafiltration module with YM 30 ultrafiltration membrane that had been pretreated with 55 PEG 4000 solution. This was done to prevent the enzyme sticking to the membrane. In anion exchange chromatography a Sepharose Q column was first equilibrated with 300ml Hepes buffer. This was made up of 200mM pH 8 with 50mM KCl. 70-80mg of protein sample was loaded and the elution was started using two different salt gradients. To prevent enzyme inactivation after elution all the fractions were titrated back to pH 7.2. All fractions that contained enzyme activity were pooled and concentrated by using ultrafiltration. Gelfiltration experiments are carried out on a prepacked HiLoad 16/60 Superdex 200 prep grade column that was connected to FPLC equipment. Four samples containing 2mg of protein were loaded and eluted with a flow rate of 1 ml min-1. The fractions were then pooled and stored at -20oC in 500 µl aliquots. (Elling and Kula., 1993) To determine the purity of the protein, SDS-PAGE is carried out. This is done by loading 100 µg of protein samples to a 125 SDS-polyacrylamide slab gel that was overlaid with stacking gel. The electrophoresis was carried out at 4oC and at 40V for 16 hours a nd followed by 200V for one hour. Coomassie blue R 250 was used to stain the gel followed by destaining. (Kumutha et al., 2008) Factors that affect Sucrose Synthase Activity: Ã…  ebkovà ¡ et al (1995) stated that sucrose synthase has two different pHs for optimal activity. In the cleavage direction it was found that most enzyme activity was observed between pH 6.0 and 8.5 at temperatures between 50 to 55oC. In the synthesis direction, a pH between 8.5 to 9.5 and a temperature of 35oC was optimal for enzyme activity. (Ã…  ebkovà ¡ et al., 1995) This would correlate with the findings of Morell and Copeland (1985) who found that optimal activity of the enzyme in soybean was at pH 6 in the cleavage direction and at a pH of 9.5, sucrose synthase activity in the synthesis direction was at its highest. It was also found that at a pH of 7.5 the cleavage and synthesis activities were their highest. (Morrell and Copeland., 1985) Elling and Kula (1995) examined the effect of buffers TES-NaOH, MOPS-NaOH, TEA-NaOH and Tris-HCl on the pH optimum of sucrose synthase activity. These were determined using UDP and TDP as substrates for the reaction. They found that the e nzyme had its highest activity in Hepes-NaOH buffer. When MOPS-NaOH and TES-NaOH buffer was used, only 60-80% activity was noted. (Elling and Kula 1995) It was also found that the velocity of the reaction could be increased by increasing the temperature where optimal activity was seen between 50 and 60oC. Xu at al (1989) reported that potato and bean are also able to withstand these high temperatures. However once the temperature goes above 60oC enzyme activity starts to decreased rapidly and was destroyed once the temperature reached 70oC. (Xu et al., 1989) The cleavage of sucrose by the sucrose synthase enzyme was investigated to find the rate of cleavage reaction using different nucleosidediphosphates as cosubstrates. They found that the rate of reaction was UDP>TDP>ADP>CDP>GDP. Echt and Chourey (1985) found similar results when examining nucleotide specificity. They found that substrate specificity for SS1 and SS2 were UDP>TDP>ADP>CDP>UTP where each substrate was at a concentrat ion of 4mM. (Echt and Chourey 1985) Low levels of heavy metal ions such as mercurate inhibited cleavage activity of the enzyme. This would lead to the assumption that sulfhydryl groups are involved in the catalytic process. It is also inhibited by Tris-HCl and by small concentrations of MgCl2 and MnCl2. (Ã…  ebkovà ¡ et al., 1995) Cations were shown by Elling and Kula (1995) to have a slight influence on enzyme activity. The activity was lessened slightly (10%) by the presence of 1mM Mn2+ and Mg2+ ions with UDP. The enzyme is completely inactivated in the presence of 1mM Cu2+ or Fe2+. (Elling and Kula., 1993) A recent study was undertaken to examine the effects of volatile emissions on carbohydrate metabolism. Studies on this area have taken place before but it is usually examining the results of physical contact between the host plant and the microbe. No work has taken place until now on the effect on the plant in the absence of physical contact. Many microbes such as Pseudomonas spp, Strepomyces spp, Penicillin spp and a selection of truffles produce ethylene. This gaseous plant hormone plays an important role in many aspects of plant growth and development such as seed germination, root hair initiation, fruit ripening and starch accumulation. In the work of Ezquer et al (2010), the possible effects of volatiles released from gram-negative bacteria, gram-positive bacteria and fungi on starch metabolism was studied. The results showed that the volatile compounds released by microbes promoted high levels of starch accumulation in mono- and dicotyledonous plants. It also revealed fungal vo latiles (FVs) promoted massive changes in expression of genes involved in many important processes in plant such as metabolism of carbohydrates, amino acids, sulphur and lipids, energy production, protein translation and stability, cell wall biosynthesis and photosynthesis. However no changes were noted in the expression in some of the genes that coded for proteins involved in starch and sucrose metabolism such as plastidial hexokinase, plastidial phosphoglucose isomerase, plastidial adenylate kinase, alkaline invertase and UDPglucose (UDPG) pyrophosphorylase. It was found in the study that FVs strongly upregulate the expression of Sucrose Synthase in potato leaves. The plants were cultured in the presence and absence of FVs emitted by A. Alternata. This caused a massive enhancement of expression of Sus4 isoform. A 29.4- and 31.63-fold increase was observed in expression when the plants were cultured in the presence and absence of sucrose. This isoform of the enzyme controls the accumulation of ADPG, UDPG and starch in potato source leaves and tubers. Analyses of the intracellular amounts of starch and nucleotide-sugars in the leaves of the plant show a positive correlation between patterns of enzyme activity and starch, UDPG and ADPG amounts. This was noted when the leaves were cultured in the presence and absence of FVs. Western blot analyses and quantitative RT-PCR confirmed also the increase in expression. (Xu et al., 1989) Environmental Factors affecting Sucrose Synthase Activity: Anoxia: Waterlogging is where oxygen supply is blocked to root leading a severe decrease in the amount of oxygen available to the plant. This leads to inhibition of root respiration that causes a major decline in energy of root cells affecting vital metabolic processes of the plant. This is restriction of oxygen supply is known as anoxia. The presence of glucose in an anoxic incubation medium drastically decreases meristem death and studies have shown that sucrose synthase is the enzyme mainly responsible for sucrose breakdown under anoxia. (Kumutha et al., 2008) The increase in glycolytic demands caused by these demands is the cause of increased sucrose synthase expression. This has been demonstrated in many plant species e.g. sucrose synthase gene is induced in wheat and in rice when oxygen levels are low. (Ricard et al., 1998) Harada et al (2005) also found an increase in sucrose synthase activity in pondweed turins while under anoxia. (Harada et al., 2005) Klotz and Haagenson (2008) foun d that sugarbeet contained two genes for sucrose synthase activity-SBSS1 and SBSS2. They demonstrated that anaerobic conditions caused a large increase in the transcription levels of SBSS1 and a quick increase and succeeding decline in SBSS2 transcription levels. However this did not correlate with a significant increase in sucrose synthase enzyme activity. A 23% increase in sucrose synthase activity was noted after initiation of anaerobic conditions but otherwise the activity of the enzyme did not differ greatly to that of the controls. (Klotz and Haagenson., 2008) Fig 11: The graph outlines the different rates of sucrose synthase activity in the con

Progressive Insurance :: Progressive Mutual Insurance Company

Abstract On March 10, 1937, Joseph Lewis and Jack Green started Progressive Mutual Insurance Company. They wanted to provide vehicle owners with security and protection and they thought an insurance company was a good investment for a couple of lawyers who were just getting started. Since its beginning, Progressive has taken an innovative approach to auto insurance. They offered drive-in claims service before any other auto insurance company and in another industry first, they allowed customers to pay their premiums in installments. An appealing option for those who could not afford annual payments. Progressive wanted and still wants to make auto insurance accessible and easy so more people could protect their vehicles. Progressive began in 1937, and over the years, they have worked hard to build a superior proposition for auto insurance consumers through competitive pricing and by continuously improving our products and services. Today, they offer competitive rates and 24-hour, in-person and online services to all drivers throughout the United States. Today, Progressive customers can purchase auto insurance directly from them online or by phone, or they can purchase from independent insurance agents. Headquartered in the Cleveland suburb of Mayfield Village, Ohio, Progressive has 28,000-plus employees in more than 450 offices throughout the country. Progressive people enjoy a casual dress code and a unique work environment where all are required to risk, learn, grow and perform. Progressive's larger locations house one of the country's largest, most eclectic contemporary art collections. Communicating a clear picture of Progressive by stating what they try to achieve, how they interact with custom ers, and what guides their behavior permits all people associated with Progressive to understand what they expect of each other and ourselves and how we conduct our business. Progressive has been in business since 1937 and currently is one of the largest auto insurance groups in the United States. They have more than 10 million policies in force and growth continues as more people choose us for their vehicle insurance needs. They sell insurance directly to customers online and by phone, and offer insurance through more than 30,000 local independent agents. In addition to auto insurance, Progressive offers the following types of insurance to customers throughout the country: boat/personal watercraft insurance, commercial auto insurance, homeowners insurance, motorcycle insurance, RV insurance and Segway insurance. Policies are available for people who choose to shop for auto insurance online at progressive.com or by phone at 1-800-PROGRESSIVE 1-800-776-4737. You also can compare rates for other top companies when you shop for a Progressive auto policy. Progressive Insurance :: Progressive Mutual Insurance Company Abstract On March 10, 1937, Joseph Lewis and Jack Green started Progressive Mutual Insurance Company. They wanted to provide vehicle owners with security and protection and they thought an insurance company was a good investment for a couple of lawyers who were just getting started. Since its beginning, Progressive has taken an innovative approach to auto insurance. They offered drive-in claims service before any other auto insurance company and in another industry first, they allowed customers to pay their premiums in installments. An appealing option for those who could not afford annual payments. Progressive wanted and still wants to make auto insurance accessible and easy so more people could protect their vehicles. Progressive began in 1937, and over the years, they have worked hard to build a superior proposition for auto insurance consumers through competitive pricing and by continuously improving our products and services. Today, they offer competitive rates and 24-hour, in-person and online services to all drivers throughout the United States. Today, Progressive customers can purchase auto insurance directly from them online or by phone, or they can purchase from independent insurance agents. Headquartered in the Cleveland suburb of Mayfield Village, Ohio, Progressive has 28,000-plus employees in more than 450 offices throughout the country. Progressive people enjoy a casual dress code and a unique work environment where all are required to risk, learn, grow and perform. Progressive's larger locations house one of the country's largest, most eclectic contemporary art collections. Communicating a clear picture of Progressive by stating what they try to achieve, how they interact with custom ers, and what guides their behavior permits all people associated with Progressive to understand what they expect of each other and ourselves and how we conduct our business. Progressive has been in business since 1937 and currently is one of the largest auto insurance groups in the United States. They have more than 10 million policies in force and growth continues as more people choose us for their vehicle insurance needs. They sell insurance directly to customers online and by phone, and offer insurance through more than 30,000 local independent agents. In addition to auto insurance, Progressive offers the following types of insurance to customers throughout the country: boat/personal watercraft insurance, commercial auto insurance, homeowners insurance, motorcycle insurance, RV insurance and Segway insurance. Policies are available for people who choose to shop for auto insurance online at progressive.com or by phone at 1-800-PROGRESSIVE 1-800-776-4737. You also can compare rates for other top companies when you shop for a Progressive auto policy.

Emily Character Analysis of A Rose for Emily Essay -- A Rose For Emily,

Miss Emily Up Close In the short story of A Rose for Emily, the main character illustrates a disturbed individual that doesn't want to separate herself from a deceased loved one. Everyone knows what its like to loose a loved one, but the town of Jefferson had no idea how hard Emily had taken death until they unraveled her deep, dark secret. People knew what it must have been like to be Emily. They knew the type of life she had lived and felt bad for the way her father had kept her all cooped up and sheltered away from any man. They also knew her father had felt that no man was good enough for her. The people of Jefferson felt that the family thought they were better then everyone else and showed no sort of insanity. When her father died, people realized the toll it had taken on Emily. They were somewhat relieved to know he was gone, and she too would discover what it was like to be a ?pauper.? They also knew they could pity her and the woman even came to her house to console her. They found it interesting when they saw no sense of grief amongst her face, and that she acted like she had no idea of the death of her father. People of Jefferson still didn?t think she was crazy, they had just figured it was all she had to do. She wasn?t very tidy and she had left an African American to clean up her house and be her butler . They women knew that a man surely didn?t know how to clean a house so they knew he was the reason for the horrible smells. The scent of her house was gruesome, and left people nauseous. Unfortunately, no one had the guts to let her know that she had basically had an odor, which surrounded her property. So the townspeople had squeezed lemon juice around her yard to relieve the horrid smell. When Miss Emil... ...dering emotions which might have driven Emily to her insanity. Although it is hard, I have not even thought of the perverted things that she had done to Mr. Barron, nor have I thought that an individual was capable of telling themselves that someone isn?t dead and argue with people and say I wouldn?t give up their body. Emily did go through a lot in her lifetime. It is hard to loose someone close to you, and especially if that is all you truly have. I feel that all Emily wanted was to feel loved, and to have someone in her life that made her feel special and feel like she was somebody. She was lonely, and she needed someone to love her and someone for her to love. She sought attention which she rarely received as a child. She demonstrates a quite luminous type of psychosis that was affected and hindered from her father?s death and corrupt and shelter childhood.

The Ultimate Rifle Essay -- Technological Innovations, Advancements

In today’s world technology is constantly changing and evolving, at the forefront of technological innovation is the United States Military. The US military has lead major break troughs with satellites, GPS, aerospace engineering, marine exploration, and of course weaponry. In recent years a major weapons manufacturer named Heckler and Koch began designing and testing a new rifle that would retire the military’s standard issued M4. The proposed weapon is the H&K XM8. This rifle is more versatile, more reliable, and designed to better suit the soldiers carrying it than the military’s standard issued M4 and because of this the XM8 will help save lives. The XM8 is one of the most versatile weapons ever created. The XM8 is capable of exchanging interchangeable assembly groups consisting of the barrel, lower receiver, hand guard, butt stock modules, and sighting system ("XM8 Lightweight Carbine." xx). That’s not all, the XM8 is also capable of under barrel attachments such as the XM320 40mm grenade launcher and the XM26 modular lightweight shotgun system which can be added in the field without tools. The XM8 allows for the exchange of these butt stocks; the standard collapsible multi-positional version, an optional butt cap for portability, or a sniper butt stock with and adjustable cheek piece (Military Gear Spotlight: XM-8 Rifle). By allowing for different stocks the XM8 is capable of maximum comfort and performance for various situations. Being uncomfortable in a stressful situation is distracting, much like having a rock stuck in your shoe or a piece of food stuck between your teeth, only now the weapon that is your first line of protection is what is distracting you and putting your life in jeopardy. The XM8 essentially ensures t... ...tp://ezproxy.wnc.edu:2312/ehost/pdfviewer/pdfviewer?sid=e8a16477-59f6-4978- 98b2-f7773ede5fcb%40sessionmgr113&vid=7&hid=112>. "LEAVE TRIGGER ASSEMBLY ALONE." PS: Preventive Maintenance Monthly. Oct. 2005: 18. Web. 14 May 14. . "Military." XM8 Lightweight Carbine. N.p., n.d. Web. 10 May 2014. . "Military Gear Spotlight: XM-8 Rifle." Military Gear Spotlight: XM-8 Rifle. N.p., n.d. Web. 10 May 2014. . "US Army Reviews OICW Programme." N.p., n.d. Web. 05 May 2014. .

Light Beer versus Dark Beer

Thesis StatementThere are several differences between light beer (which include the following: American Standard Beers, American Light Beers, American Wheat Ales, Belgian Wheat Ales, Hefeweizen, and Pilsners) and dark beer (which include Brown Ales, German Bocks, Nut Brown Ales, Porters, and Stouts). Some of the differences include their origins, the processes involved in making or producing it, as well as, their popularity since light beer is far more popular than dark beer.OriginBoth light and dark beer came to exist in 1772 (Chronology.. n.p.). The production of both began in 1587 when the immigrants of Virginia brewed ale through the use of corn (Chronology.. n.p.). Then twenty years after that, England sent its first shipment of beer to the immigrants of Virginia (Chronology.. n.p.). Two years after that, London were already looking for brewers to assist those immigrants of Virginia; back then advertisements which states, â€Å"American Help Wanted† were posted (Chronolog y.. n.p.). After that, the first brewery has been instituted in Manhattan by â€Å"Adrian Block and Hans Christiansen† (Chronology.. n.p.).The institution of that brewery aforementioned caused the birth of â€Å"Jean Vigne† who was the â€Å"first non-native American to become the first brewer horn in Manhattan† (Chronology.. n.p.). In addition to that, after a few years, â€Å"Governor Van Twiller† along with the â€Å"West India Company† built another brewery in New Amsterdam on Brewers Street (Chronology.. n.p.). This has been followed by another brewery which was built on Manhattan Island by Peter Ninuit (Chronology.. n.p.). After so many breweries have been instituted, a license to open a pub in Boston has been awarded to a man named Samuel Cole (Chronology.. n.p.).Three years later, the â€Å"first authoritatively recorded brewery in the Massachusetts Bay Colony under the control of Captain Sedgwick has been instituted† (Chronology.. n .p.). Then another brewery has been built located in Providence, Rhode Island which has been assigned and to be led by Sergeant Bauleton (Chronology.. n.p.). Immediately following that, â€Å"Samuel Wentworth has been given a license to operate and brew beer in New Hampshire† in 1670 (Chronology.. n.p.). Two more breweries were set up: one in 1683 which was set up near Bristol, Pennsylvania and another one in 1738 which has been established in Jekyll Island, Georgia (Chronology.. n.p.). Then finally after 34 years, dark and light beer have been concocted in England (Chronology.. n.p.).Process of Making ItIn making light beer, the first stage is to â€Å"steep the grain in water until it sprouts or germinates† (SF Brewers Guild.. n.p.). After that stage technically referred to as â€Å"malting†, the grain/malt is then heated and dried â€Å"in order to stop the malt from germinating further† (SF Brewers Guild.. n.p.) After that process known as â€Å"kiln ing†, the malt may be roasted further according to preferred color and flavor (SF Brewers Guild.. n.p.). The next step is to â€Å"process the dried malt in a mill in order for the outer coating of the grain to crack† (SF Brewers Guild.. n.p.).After cracking it will placed in a container then it will be poured with hot water (SF Brewers Guild.. n.p.). Here, the sugars contained in the grain will be broken down and released in the water and will consequently produce a sweet liquid called wort as well (SF Brewers Guild.. n.p.). After that the â€Å"wort† will be boiled for two hours and hops will be added (SF Brewers Guild.. n.p.).In making dark beer, the same processed is followed except that hops in dark beer are boiled 30 minutes or more longer (SF Brewers Guild.. n.p.).PopularityIt is said that college students are more likely to drink light beers. This is simply because it is easier to drink than dark ones. However, a medical breakthrough affected such popular ity of light beer because dark beer has been proven to prevent blood clots (Peck n.p.). In addition to that, it has â€Å"powerful antioxidant effects† (Fauber n.p.).ConclusionBoth light and dark beers were produced in 1772 sharing the same history. They differ, however in the process of making it since dark beers involve more time in boiling hops than in light beers. Also, in terms of popularity, although dark beers are proven to be good for the health, more people still go for light beers especially college students.ReferencesChronology of the American Brewing Industry. n.d. n.a. 22 October 2007http://www.beerhistory.com/library/holdings/chronology.shtmlFauber, John. Dark Beer Better for Heart. 11 November 2003. The Temple News.22 October 2007http://temple-news.com/wp-signup.php?new=media.wwwPeck, Peggy. Dark Beer May be Better for the Heart. 11 November 2003. WebMD.22 October 2007http://www.webmd.com/diet/news/20031111/dark-beer-may-be-better-for-heartSF Brewers Guild. How Beer is Made. n.d. n.a. 22 October 2007http://sfbrewersguild.org/beer.html

Describe the characteristics and cause of challenging behaviour Essay

Case study 1 In case study 1 Winston is physically aggressive. However over recent months he has become very withdrawn, extremely grumpy and agitated. In the case study it says how Winston wife known as Ruby was assisting him to get ready for bed when he lasted out punching her in her chest. This shows he has become physically aggressive. The characteristics for his behavioural change, was that he shouts obscenities loudly, especially in the evening before going to bed. The cause of his behaviour is due to the fact he had Alzheimer disease. 1. What type of challenging behaviour is being displayed? The challenging behaviour being displayed is reactive. 2. How do you know this? Describe the characteristics His characteristic of his challenging behaviour is intimidating, aggressive and abusive. I know this because it says in the case study that Winston ‘shouts obscenities loudly, especially in the evening before going to bed, and has now become physically aggressive towards his wife’ another stage that shows his challenging behaviour is when Ruby is assisting him and he lashes out punching her in the chest. 3. What are the causes or triggers?  Winston has Alzheimer Disease. This is a type of dementia. Dementia is a group of symptoms which affects the way the brain functions; this could also affect the way Winston behaves as he could become aggressive. The cause of Winston’s anger is due to his physical illness, (Alzheimer Disease) this makes it difficult for him to do some activities and that’s why he has the help of his wife. Case study 2 Sean’s 21 years old and has been living with his auntie for the past two months because life at home has been very uncertain due to his parents going through a divorce. He does not know his auntie very well and is slightly uncomfortable to talk to her about his personal issues. Sean regularly attends youth club and enjoys meeting his friends from school there. She asks him about his career history and he doesn’t seem to like it as he responds abruptly. He suddenly appears to be very offended by the questions and starts to swear at the support worker repetitively and says ‘what do you need to know this for?’ He gets up and says ‘Forget it’. He starts to walk away while shouting racist comments towards the support worker. 1. What type of challenging behaviour is being displayed? In the case study it says Sean’s, ‘starts to walk away while shouting racist comments towards the support worker.’ This shows that his behaviour is challenging and he shows this verbally. 2. How do you know this? Describe the characteristics I know this because when Sean’s support worker talks to him, he’s response with a short reply, he swears at the support worker over and over asking why the support worker is questioning him. Sean characteristics are intimidating and violent. 3. What are the causes or triggers?  Sean’s behaviour is could be caused due to the fact his mother and father are separated and divorced. Because of this, he may feel unhappy and stressed. When his support worker questions him about his employment history he might have been worried about being judged.

The Evolution of English Churches

The development of English churches The English churches which are emerged during the epoch of different reigns and its architectural features are a alone country of survey. It varies in many facets from other manners of architecture every bit good as the stuffs used. The period from 600 AD to 1900 AD was the period in which the different architectural manners were emerged. It starts with the Anglo Saxon period to Victorian period. It is a huge country of survey in which the picks of construction were selected based on many grounds like influence of households or swayers, handiness of stuffs etc. Each and every period was landmarks of the architecture which influenced in the building of English churches within the cultural boundaries. During the reign of different emperors, the fortunes for doing different assortments in the building of churches became a tendency and it created different architectural manners. Every manner has difference in many facets and the workers followed the forms in similar mode on all the buildings in that period. Anglo Saxon period The Anglo Saxon period started from 600 AD to 1066 AD. The plants which carried out during this period depicts the influence of Roman business in the Britain. Later it became the far making manner of architecture spread to some other parts of Europe. The relatively geographical isolation, the distribution and handiness of edifice stuffs and workingmans contributed to this manner of British architecture which we call now as Anglo Saxon period. The influences can be seen particularly in the Basilica program, the Celtic program, etc. All the earliest churches which were built here whilst Britain belonged to the Roman Empire and in the southern parts where the influence of the Christianity predominated for long afterwards were in the basilican manners. Outside the southern England these were seldom found during the terminal of 7th century. The digging work which was carried out at the Roman town of Silchester, Hampshire, revealed a 4th century Christian church of this type. Augustine’s foremost cathedral at Canterbury has similar characteristics of St Peters basilica at Rome. Each Anglo Saxon church had its ain distinctive characteristics which is the ground the current province of each church is different other than its land program. The churches were low, unpretentious and purely functional. That is the difference of Anglo Saxon edifices with the replacements. The best sanctum crosses are done with rock in this period which are shown as an illustration of early Christian art in the northern portion. The favorite subjects of the Anglo Saxons were animate beings, birds, foliages, vines and scriptural characters. The workers chiefly worked with cocks and chisels. The church walls look strong and solid though they were non truly thick. Quoining was another characteristic of the Anglo Saxon walls in which the rocks will be placed on top of the other at the point where two walls met. Towers at this period were square, rectangular or round in form. It reaches a upper limit of 70 pess. They were non professional in building techniques that they could construct traversing towers on pies and arches. The Windowss were little and the church was affected by conditions jobs. Glass was available but it was really expensive because of its foreign beginning. Most of the Windowss were directly sided and unit of ammunition headed. There were cells built under the organic structure of the church which is called as the crypts. These were found in the edifices which were built by the well traveled missionaries. This was a convenient topographic point to hive away, exhibit and were besides used to conceal hoarded wealths or sacred relics which were collected during the pilgrim's journey. The crypts walls were thick and were divided into differentiated countries. These were the peculiar characteristics which were subsequently became treatments and hallmarks of the Anglo Saxon architectural manner. During its broad heritage of more than 5 centuries the manner made its grade in the history of British church building every bit good as the overall architecture of the British edifices, though it varied in assorted countries of England. The Norman and Transitional period These were another noticeable period which played a major function in doing the elegant manner of the British architecture. The Norman period extended from 1066 AD to 1160 AD and the Transitional period was 1150 AD to 1200 AD. Like the Anglo Saxons, the architecture in Normandy where they developed a manner of Romanesque which has its ain regional and local features. Edward the Confessor made the Norman architecture implemented in the ecclesiastical affairs. The Council of London, 1075, moved dioceses to big Centres and at that topographic point cathedrals were started in Norman manner. This tendency was ended by the male monarch William II who made great demands on the church architecture. Until 12th century the Anglo Saxon church art and sculpture was far advance than the Norman architecture. The first church which is built in Norman manner after the conquering of were solid looking and solemn, and was virtually barren of any coloring material or ornament. Norman architecture was developed in the late 30 old ages of the 11th century as it did in Normandy, but the church edifice rushed in front. The overall thought was to supply every small town with a topographic point of worship and as a consequence every medieval church will stay Norman in beginning. The ornamentations were unusual including the beak caput which was a favorite signifier of ornament, which take us into which is used in adorning truss tabular arraies. The caputs of firedrake, birds, animate beings etc were given a long beak or pointed mentum which extended over a convex modeling to turn over below. The Norman walls were holding rubble infilling between the teguments of appareled rocks and were looking midst and solid. The faces had shots which were made during the carving of the axe. Buttresss were introduced in this period which was meant chiefly for projecting from land degree against the exterior wall, in contrast to the Saxon pilasters which appeared at many points on wall surfaces. They were rather apparent built in one phase with upper surfaces inclining towards the corners. The towers were no longer provided as the chief entryway to the church, whereas it is used as the domination portion of the church which is noticeable than anything in the environment. The Normans developed a technique for back uping the cardinal tower with wharfs which is placed merely at the angles, which became the best advantage of the tower design. Towers were fundamentally built in rectangular or square base but may be built round where there is a deficiency of stuffs. The Windowss were extremely setup carbon monoxide related with the exterior wall surface, but were used as a natural visible radiation beginning. The doors were decorated with sanctuary arches which were the most dramatic characteristic of the eleventh and 12th century architecture. The perpendicular boards of oak were used as the door elements which was battened horizontally and press sets were given outdoors. The chief designs used by the Normans for their founts were round unmounted, round mounted on a cardinal root with several other supports which his either used for functional capacity whereas the square unmounted is normally thick cornered and is holding cardinal root. They carved the objects which they were most afraid of every bit good as the objects they like the most with lasting stuffs. In this period legion cosmetic thoughts were developed which was begun with petroleum carving and a figure of regional manners were evolved. In the transitional period the builders and the Masons were seeking for new ways of showing their development accomplishments. The usage of axe was replaced by chisels and cocks which made a new tendency and gave good consequences. The first transitional pointed arches were constructional which was non seen earlier. Sometimes the unit of ammunition headed arches and the pointed arches were placed side by side in such a manner that one can see how short a infinite of clip that latter took consequence. The highs of towers in this period increased and the gaps which were round in form besides contained some pointed arches. The transitional period may be otherwise called as the transmutation period from Norman to the Early English period. The Early English period It was the period in which the Victorians liked the least but for which they accorded the descriptive term ‘first pointed’ was curious to England in the old ages between Romanesque and geometrical Gothic. This period extended from 1200 AD to 1300 AD and was a glorious age where the British ecclesiastical plants started to do its English manner. In this period the barbarian enthusiasm in the architectural positions and beliefs were dominated by the echt spiritual ideas. It was influenced by the reigns of Richard ( 1189-99 ) and John ( 1199-1216 ) and their ideas made much alteration in the buildings at that clip. By the starting of 13th century the church has become rectangular or cruciform in its land program. The builders have got an thought how to manage burden and structural strains by the clip of this period in 13th century. They did experiments on the burden facets and a new assortment in the building techniques was created. The most abiding design in this period was crocket. The design was influenced by the classical capitals of abroad which was altered and made in the concave hook form with shafts and had a lobbed visual aspect. On the borders of arches and goons the Canis familiaris caput was sculptured and was the precursor of the designs in the 14th century. In some countries the churches were little and tower less. The wall doesn’t have much greater thickness to maintain them up and back up the weight from above. The walls still had a rubble masonry construction but the cognition gained by the Masons helped to better a batch in the structural parametric quantities. The realization of the mistakes in the old building plants made noticeable alteration in the walls and even in the arrangement of window. The form of window was changed to indicate headed and it extended to the tracery. The buttresses projected more from the wall than earlier and were extended above the land degree to an extent. They were largely straight sided and the borders were chamfered. The twine classs were little and elegant which can be seen throughout the edifice and had about tantamount characteristics which they were resembled. The Decorated The first Edwardian epoch was another aureate period which the Victorian loved the most for its pureness. They called this period as in-between or 2nd pointed when the church architecture resembled the Gallic constructions in the 14th century. This period started by the terminal of 13th century, to be specific 1300 AD and ended at 1377 AD. Though it has similar characteristics of Gallic manner of architecture, it doesn’t peculiarly resemble that in the concluding phase. The imaginativeness and wild thought of the emperors every bit good as the creative persons made the churches more adorned than earlier. They included more seats to the church and made it more secular than earlier. Inside the church societal events took topographic point and became more unfastened to the populace. The internal walls were coloured decently and they covered more with cosmetic elements, whereas the services were made of ritual. Overall cosmetic result of this clip was ne'er been realised. During this clip the building of many churches remained unfinished because of decease of Masons and builders due to blight. Even though these calamities happened, the term decorated genuinely depicted in largest churches. There was non much promotion in the program or executing of the church edifice, but the basic programs of aisled nave, sanctuary, western tower, north and south porch had great alterations in 14th century. Largely the new ornaments were seen in the outside. Large Windowss were inserted in to the sanctuary walls. Normally a big church was constructed with three floors named arcade, triforia and clearstory, whereas in smaller 1s the in-between bed was omitted. There were legion moldings in the 14th century, but were much less undercut than earlier. Crockets became more attractive as they implemented a natural incurvate manner. Interior walls became more decorated in coloring material, whereas the surfaces were non prepared good. Another characteristic was the cyma reversa arch which was applied every bit widely as possible. Buttresss achieved the maximal flawlessness in footings of its proportions every bit good as cosmetic beauty. They were wider than earlier and were holding more jutting visual aspect at the base. Roofs were largely built in lumber and were covered with rock tiles, lead or herpes zosters. Builders tried better ways of structural agreements which was another advantage of this period. It was non until this period and even so none excessively shortly in most countries, that full advantage began to take topographic point of the clearstory, and it became a standard characteristic. Builders started adding parapets which were better treated and has became a major component of ornament. Although some octangular towers were put up, two, three or four phase square program was largely preferred. Cornice was decorated with ball flower, rolling four leaf ornament or little caputs. Windows became another separating characteristic of the church in both figure and size, in a proportion to the available sizes. Windows of little churches had four visible radiations and may widen up to 9 in larger edifices. The outside porches were made reasonably apparent with its roofing at sensible highs and exterior gaps similar to interior room accesss. They may or may non incorporate parapet walls. But will be decorated with heraldry. Some of them were unfastened work lumber porches which were built on rock or wooden base. Doorways became more pointed whereas it varied in size. Pedestals were made in different phases like unit of ammunition, square or octangular with smaller base country. Another flowery illustration was the sides of the bowl which was divided by either the buttress or shaft, which were topped by pinnacles. The base of these was frequently on caputs or knots. Perpendicular age This age was between 1377 AD and 1547 AD when a curious English manner of church architecture was born. This period was the longest in the history than any other periods and had batch of single typical characteristics. In parish churches this tendency was executed by Masons who were non the best but who could get by with its simple lines. They had elegant manners which had beautiful proportioning. The basic perpendicular run alonging continued to widen throughout the edifice. When compared to the cosmetic age, the plants were looking plainer and the inside of the churches became colder. The form of the shadiness, shadow and visible radiation was non affected for the sculpture detailing and Masons concentrated in doing big Windowss in high walls. The shell of the church had become a topographic point for puting dominant characteristics which can be given as an illustration for originative excellence. There was an astonishing rise for the wood worker whose plants were considered every bit as Masons. Voluntary administrations came for supplying community services in the 14th century. Some of them were rich trading administrations who have the capacity to construct their ain chapels or take over a portion of church. The program of the church was made alterations harmonizing to the demands of clubs and persons for chapels and chantries. The nave walls which had protruded from the roof were taken down and reconstruct to include a clearstory. This became an ineluctable standard characteristic of perpendicular churches. Surface was decorated widely where some twine classs were omitted in small town churches smaller in size. Dripstones or label Michigans were returned at the springing of the arch, which were on occasion curved towards the wall and embattled. Walls were built high and was constructed of skillfully shaped ashlars, but weakened in themselves by the extent to which the surface country was reduced to a lower limit in order to suit big Windowss. Spouts were provided for transition of H2O from the parapet keeping. George masons experimented with different parapets and a great assortment of parapets were introduced in this clip. The parapet was constructed in elaborate ornamentation spliting into three parts named get bying set and valance. One of the disadvantages of the dilutant walls was to take slender buttresses still from the walls surface. Diagonal buttresses climbed the tower and corresponded with the phases, be givening to decease into the wall merely below the parapet. Flying buttress was non widely accepted in the state and was on occasion put up the whole length of the nave. The most beautiful roofs were built unfastened and appeared high and broad, had an absence of tie beam. The cock beam roof was considered sole for the eastern states. It support arch bases widening from the horizontal stations to the church caput. Sometimes the larger churches tried to hold a dual cock beam roof and they remained the richest among the extremely decorated 1s. The complicated vaulting was experimented during this period even though it was relatively rare.